解剖学和形态学
麻醉学
听力与言语-语言病理学
行为科学
心脏和心血管系统
细胞和组织工程学
临床神经病学
危重症监护医学
牙科,口腔外科和医学
皮肤病学
急诊医学
内分泌学和新陈代谢
肠胃学和肝脏学
老人病学和老年医学
卫生保健科学和服务
血液学
免疫学
传染病
综合和补充性医学
医学伦理学
医学信息学
医学实验室技术
医学,全科和内科
医学,法律
医学,研究和试验
神经系统科学
护理
营养学和饮食学
产科医学和妇科医学
肿瘤学
眼科学
整形外科学
耳鼻喉科学
病理学
儿科学
周围血管疾病
药理学和药剂学
生理学
基本医疗保健
精神病学
公共、环境和职业卫生
放射学,核医学和医学成像
康复学
生殖生物学
呼吸系统
风湿病学
运动科学
外科学
毒理学
热带医学
泌尿学和肾脏学
病毒学
老年医学
健康政策和服务
心理学,临床
abstract::One nanosecond molecular dynamic (MD) simulation of anti-hen egg white lysozyme (HEL) antibody HyHEL63 (HH63) complexed with HEL reveals rigid and flexible regions of the HH63 binding site. Fifty conformations, extracted from the MD trajectory at regular time intervals were superimposed on HH63-HEL X-ray crystal struc...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章
doi:10.1385/CBB:43:2:253
更新日期:2005-01-01 00:00:00
abstract::We apply methods from statistical physics (histograms, correlation functions, fractal dimensions, and singularity spectra) to characterize large-scale structure of the distribution of nucleotides along genomic sequences. We discuss the role of the extension of noncoding segments ("junk DNA") for the genomic organizati...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章
doi:10.1385/CBB:42:2:145
更新日期:2005-01-01 00:00:00
abstract::In 1903, George Hulett explained how solute alters water in an aqueous solution to lower the vapor pressure of its water. Hulett also explained how the same altered water causes osmosis and osmotic pressure when the solution is separated from liquid water by a membrane permeable to the water only. Hulett recognized th...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章,评审
doi:10.1385/CBB:42:3:277
更新日期:2005-01-01 00:00:00
abstract::Coronary blood flow is tightly coupled to myocardial oxygen consumption to maintain a consistently high level of myocardial oxygen extraction. This tight coupling has been proposed to depend on periarteriolar oxygen tension, signals released from cardiomyocytes (adenosine acting on K+(ATP) channels), and/or the endoth...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章,评审
doi:10.1385/CBB:43:1:017
更新日期:2005-01-01 00:00:00
abstract::The transforming growth factor (TGF) superfamily encompasses about 30 members in mammals. The effect of TGF-beta subfamily members is exerted and regulated via selective pathways of synthesis and signaling that involve activation of latent TGF-beta, specific and high-affinity binding to cell membrane serine/threonine ...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章,评审
doi:10.1385/CBB:43:1:131
更新日期:2005-01-01 00:00:00
abstract::Tissue restoration is the process whereby multiple damaged cell types are replaced to restore the histoarchitecture and function to the tissue. Several theories have been proposed to explain the phenomenon of tissue restoration in amphibians and in animals belonging to higher orders. These theories include dedifferent...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章,评审
doi:10.1385/CBB:40:1:1
更新日期:2004-01-01 00:00:00
abstract::Discovered roughly 10 yr ago, Jak2 tyrosine kinase has emerged as a critical molecule in mammalian development, physiology, and disease. Here, we review the early history of Jak2 and its role in health and disease. We will also review its critical role in mediating cytokine-dependent signal transduction. Additionally,...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章,评审
doi:10.1385/cbb:41:2:207
更新日期:2004-01-01 00:00:00
abstract::Kaposi's sarcoma-associated herpesvirus (KSHV), also known as human herpesvirus 8, has been associated with the development of Kaposi's sarcoma, pleural effusion lymphoma, and multicentric Castleman's disease. KSHV is a double-stranded DNA virus that has been classified as a gammaherpesvirus. The viral genome is appro...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章,评审
doi:10.1385/CBB:40:3:305
更新日期:2004-01-01 00:00:00
abstract::Recent spectroscopic studies of photosynthetic light-harvesting supramolecular complexes at the single supramolecule level are reviewed. This report describes the "single-molecule" investigation on light-harvesting complex 2 (LH2) of purple photosynthetic bacteria, phycobiliproteins of cyanobacteria and red algae, lig...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章,评审
doi:10.1385/CBB:40:2:149
更新日期:2004-01-01 00:00:00
abstract::Tetrahydrobiopterin (BH4) is a member of the pterin family that has a core structure of pyrazino-2,3-d-pyrimidine rings. Because BH4 is an essential cofactor for the biosynthesis of nitric oxide (a major vasodilator), there is growing interest in BH4 biochemistry in endothelial cells (the cells that line blood vessels...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章,评审
doi:10.1385/CBB:41:3:415
更新日期:2004-01-01 00:00:00
abstract::The American Diabetes Association announces funding for clinical and basic science studies in three major areas of research that focus on cell-based insulin replacement in type 1 diabetes: 1. Genetic engineering of nonpancreatic cells into glucose-sensitive insulin-producing cells; 2. Transforming stem cells, pancreat...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章
doi:10.1385/cbb:40:3s:023
更新日期:2004-01-01 00:00:00
abstract::Insulin, the major secreted product of the beta-cells of the islets of Langerhans, is initially synthesized as a precursor (preproinsulin), from which the mature hormone is excised by a series of proteolytic cleavages. This review provides a personal narrative of some of the key research projects leading to the identi...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章,评审
doi:10.1385/cbb:40:3:143
更新日期:2004-01-01 00:00:00
abstract::Recent advances in the use of the atomic force microscope (AFM) for manipulating cell membranes and membrane proteins are reviewed. Early pioneering work on measurements of the magnitude of the force required to create indentations with defined depth on their surfaces and to separate interacting pairs of avidin-biotin...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章,评审
doi:10.1385/CBB:39:3:257
更新日期:2003-01-01 00:00:00
abstract::Cell membranes consist of a fluidic medium of lipids and proteins that organize into specific submicron scale structures for signaling and molecular trafficking processes. These organized molecular assemblies form as a result of the structure and chemistry of the membrane components as well as the interactions of thos...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章,评审
doi:10.1385/CBB:39:2:145
更新日期:2003-01-01 00:00:00
abstract::Leukocyte transendothelial migration is a multistep process coordinated by chemokine receptors, integrins and cell adhesion molecules. The interaction between leukocytes and endothelial cells is accompanied by bidirectional signaling in both cell types, which is initiated following formation of specialized, "docking" ...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章,评审
doi:10.1385/cbb:38:3:305
更新日期:2003-01-01 00:00:00
abstract::A recently developed method for surface modification, layer-by-layer (LbL) assembly, has been applied to silicone, and its ability to encourage endothelial cell growth and control cell growth patterns has been examined. The surfaces studied consisted of a precursor, with alternating cationic polyethyleneimine (PEI) an...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章
doi:10.1385/CBB:38:2:103
更新日期:2003-01-01 00:00:00
abstract::A method is presented to separate rabbit cardiac ventricular myosin isoenzymes (V(1), V(2), V(3)), which are large and important contractile proteins. This polyacrylamide gel electrophoresis--using a slab minigel format--does not involve preparation of an acrylamide gradient or denaturing conditions. The isoenzyme mig...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章
doi:10.1385/CBB:38:1:33
更新日期:2003-01-01 00:00:00
abstract::P2Y receptors are classified as P2 purinergic receptors that belong to the superfamily of G-protein coupled receptors. They are distinguishable from P1 (adenosine) receptors in that they bind adenine and/or uracil nucleotide triphosphates or diphosphates depending on the subtype. Over the past decade, P2Y receptors ha...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章,评审
doi:10.1385/CBB:39:1:75
更新日期:2003-01-01 00:00:00
abstract::We have studied the mechanisms of NaCl transport in the mammalian proximal tubule. Studies of isolated brush-border membrane vesicles confirmed the presence of Na+-H+ exchange and identified Cl(-)-formate and Cl(-)-oxalate exchangers as possible mechanisms of uphill Cl- entry. We found that formate and oxalate each st...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章,评审
doi:10.1385/CBB:36:2-3:147
更新日期:2002-01-01 00:00:00
abstract::The epithelial Na/Pi cotransporter (NaPi-II) is instrumental in maintaining phosphate (Pi) homeostasis in vertebrates. Hormones and metabolic factors (PTH, Pi availability) that acutely influence renal Pi excretion have been demonstrated to target NaPi-II expression. Upon stimulation, newly synthesized transporter mol...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章,评审
doi:10.1385/CBB:36:2-3:241
更新日期:2002-01-01 00:00:00
abstract::Azurin, a small blue copper protein from the bacterial species Pseudomonas aeruginosa, is mostly a beta-sheet protein arranged into a single domain. Previous folding studies have shown that the equilibrium denaturation of the holoprotein follows a two-state process; however, upon removal of the copper, the denaturatio...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章
doi:10.1385/cbb:36:1:19
更新日期:2002-01-01 00:00:00
abstract::The precise regulation of both the magnitude and the duration of Janus kinase (JAK) catalytic activity is essential for the cytokine orchestration of many biological processes, and the dysregulation of JAK activity has pathological implications. Immunosuppressive disease states, such as X-linked severe combined immuno...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章,评审
doi:10.1385/CBB:34:1:17
更新日期:2001-01-01 00:00:00
abstract::Slow inactivation in voltage-gated sodium channels is a biophysical process that governs the availability of sodium channels over extended periods of time. Slow inactivation, therefore, plays an important role in controlling membrane excitability, firing properties, and spike frequency adaptation. Defective slow inact...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章,评审
doi:10.1385/CBB:35:2:171
更新日期:2001-01-01 00:00:00
abstract::By using a functional approach of reconstituting detergent-solubilized membrane proteins into liposomes and following their function in patch-clamp experiments, we identified a novel mechanosensitive (MS) channel in the thermophilic cell wall-less archaeon Thermoplasma volcanium. Sodium dodecyl sulfate polyacrylamide ...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章
doi:10.1385/CBB:34:3:321
更新日期:2001-01-01 00:00:00
abstract::Mutations of the retinoblastoma tumor suppressor, pRb, or its cyclin-cyclin-dependent kinase (CDK) regulatory kinases or CDK inhibitors, allows unrestrained E2F activity, leading to unregulated cell cycle progression. However, overexpression of E2F-1 also sensitizes cells to apoptosis, suggesting that targeting this p...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章
doi:10.1385/CBB:33:2:199
更新日期:2000-01-01 00:00:00
abstract::Peroxisomal and mitochondrial carnitine acetyltransferase (CAT; EC 2.3.1.7) isozymes are synthesized from the first and second ATG codons of the open reading frame of one gene, Candida tropicalis CAT. Primer extension analysis and RNase protection assay revealed that the peroxisomal CAT, initiating at the second AUG c...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章
doi:10.1385/cbb:32:1-3:139
更新日期:2000-01-01 00:00:00
abstract::Hepatocyte growth factor (HGF) has been known to enhance the growth of normal hepatocytes, but also to inhibit the growth of neoplastic cells. This article examines the involvement of HGF in the hepatocarcinogenesis caused by peroxisome proliferators (PPs). Up to 78 wk after male F-344 rats were orally given (4-chloro...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章
doi:10.1385/cbb:32:1-3:221
更新日期:2000-01-01 00:00:00
abstract::The n-alkane-assimilating diploid yeast, Candida tropicalis, possesses two acetoacetyl-CoA thiolase (Thiolase I) isozymes encoded by one allele: peroxisomal and cytosolic Thiolase Is encoded by both CT-T1A and CT-T1B. To clarify the function of peroxisomal and cytosolic Thiolase Is, the site-directed mutation leading ...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章
doi:10.1385/cbb:32:1-3:285
更新日期:2000-01-01 00:00:00
abstract::Nutritional changes can affect either the assembly or disassembly of yeast peroxisomes. In the past decade, insights regarding the molecular mechanisms of peroxisome assembly have been gained chiefly through the cloning of the PEX genes obtained by complementation of corresponding pex mutants in several yeast strains ...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章,评审
doi:10.1385/cbb:32:1-3:51
更新日期:2000-01-01 00:00:00
abstract::Promotion of apoptosis (which is frequently dependent on functional p53) is thought to be critical for the effectiveness of chemotherapy or radiotherapy. Studies in this as well as other laboratories have demonstrated that breast tumor cells are relatively refractory to apoptosis in response to modalities that induce ...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章,评审
doi:10.1385/CBB:33:1:19
更新日期:2000-01-01 00:00:00
abstract::Na,K-ATPase (EC 3.6.1.37, Na,K-ATPase) is a fundamental vital membrane transport and receptor system which, after biosynthesis, is exported to the plasma membrane in inside-out vesicles. Na,K-ATPase can be extracted form the natural membrane and inserted into artificially formed phosphatidylcholine vesicles (liposomes...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章
doi:10.1007/BF02738123
更新日期:1999-01-01 00:00:00
abstract::Cellular responses to external signals often reflect alterations in gene expression. The activation of cell surface hormone or growth factor receptors upon the binding of appropriate ligands mobilizes signal transduction cascades that can ultimately impact the activity of defined sets of transcription factors. The int...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章,评审
doi:10.1007/BF02737882
更新日期:1999-01-01 00:00:00
abstract::Structural organization of the cytoplasm by compartmentation is a well established fact for the eukaryotic cell. In prokaryotes, compartmentation is less obvious. Most prokaryotes do not need intracytoplasmic membranes to maintain their vital functions. This review, especially dealing with prokaryotes, will point out ...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章,评审
doi:10.1007/BF02738242
更新日期:1999-01-01 00:00:00
abstract::The state of crosslinking of microfilaments and the state of myosin-driven contraction are the main determinants of the mechanical properties of the cell cortex underneath the membrane, which is significant for the mechanism of shaping cells. Therefore, any change in the contractile state of the actomyosin network wou...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章
doi:10.1007/BF02737896
更新日期:1998-01-01 00:00:00
abstract::cDNAs encoding two PDE-3 or cyclic GMP-inhibited (cGI) cyclic nucleotide phosphodiesterase (PDE) isoforms, RPDE-3B (RcGIP1) and HPDE-3A (HcGIP2), were cloned from rat (R) adipose tissue and human (H) heart cDNA libraries. Deletion and N- and C-terminal truncation mutants were expressed in Escherichia coli in order to ...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章
doi:10.1007/BF02737830
更新日期:1998-01-01 00:00:00
abstract::Cyclic ADP-ribose (cADPR) is a cyclic metabolite of NAD+ synthesised in cells and tissues expressing ADP-ribosyl cyclases. Although it was first discovered in sea-urchin egg extracts as a potent calcium mobilizing agent, subsequent studies have indicated that it may have a widespread action in the activation of calciu...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章,评审
doi:10.1007/BF02738307
更新日期:1998-01-01 00:00:00